A gas chromatography-mass spectrometry (GC-MS) method to determine eight anabolic steroids (diethylstilbestrol, methyltestosterone, norethindrone, 17α-ethynylestradiol, estradiol, 6α-methyl-17α-hydroxy-progesterone, estradiol benzoate, and chlormadinone acetate) was developed. Muscle samples were extracted with liquid-liquid extraction and clean-up was performed in two steps, the extracts obtained were derivatized with heptafluorbutyric (HBF) anhydride and analyzed by GC-MS. In the above method, the linear scope was -50 μg kg −1 . The range of the recoveries was –148% for diethylstilbestrol, –109% for methyltestosterone, –% for norethindrone, –120% for 17α-ethynylestradiol, –103% for estradiol, –% for 6α-methyl-17α-hydroxy-progesterone, –104% for estradiol benzoate, and –% for chlormadinone acetate. The range of the coefficients of variation within batches was –12%; the range of the coefficients of variation between batches was –11%. The limit of detections and the detection capability were and μg kg −1 for diethylstilbestrol, and μg kg −1 for methyltestosterone, and μg kg −1 for norethindrone, and μg kg −1 for 17α-ethynylestradiol, and μg kg −1 for estradiol, and μg kg −1 for 6α-methyl-17α-hydroxy-progesterone, and μg kg −1 for estradiol benzoate, and and μg kg −1 for chlormadinone acetate, respectively. These results showed that the method was widely available, accurate, and sensitive.
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